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Image Search Results


Journal: STAR Protocols

Article Title: Using the single-cell imaging system and orthotropic footpad injection to establish mouse models for experimental and spontaneous melanoma metastasis

doi: 10.1016/j.xpro.2023.102349

Figure Lengend Snippet:

Article Snippet: Super cut iris scissors, straight, 12.5-cm long , Fisher Scientific , N/A.

Techniques: Recombinant, Software, Inverted Microscopy, Injection, Sterility

Journal: MethodsX

Article Title: RELi protocol: Optimization for protein extraction from white, brown and beige adipose tissues

doi: 10.1016/j.mex.2019.04.010

Figure Lengend Snippet:

Article Snippet: Reagents/tools: , Tissue Collection • Dry ice • Tweezer #5, stainless steel, 11 cm long (Kent scientific corporation, catalog # INS600095) • Dissecting scissors, straight, 10 cm long (Kent Scientific Corporation, catalog # INS600393) • Iris forceps, serrated, 10 cm long (Kent Scientific Corporation, catalog # INS650915) • 60 mm dish (Thermo Scientific, catalog #130181) • 2.0 mL capped tubes (1 per tissue to collect) • Ethanol (EtOH) 70% Tissue Lysis • Analytical balance • Fastprep FP120 (SAVANT Instrument Inc) • 1.5 mL capped tubes (4 per tissue to extract) • Ice • Carbon Steel Surgical Blade (Bard-Parker, catalog #311121) • RIPA Buffer (10x) (Cell Signaling Technologies, catalog #9806S) • Distilled water • Protease inhibitor cocktail (Sigma-Aldrich, catalog #P8340) • Phosphatase inhibitor cocktail (Sigma-Aldrich, catalog #P5726) Centrifugation and removal of excess lipids • 0.1–10 μl, 2–20 μl, 20–200 μl, and 200–1000 μl micropipettes • 0.1–10 μl, 2–20 μl, 20–200 μl, 200–1000 μl pipette tips • Cooling centrifuge Protein quantification and sample preparation • TECAN Infinite M1000 Pro (ThermoFisher Scientific) • Microtest plate 96 well (Sarstedt AG & Co, catalog #82.1581) • BCA kit (Sigma-Aldrich, catalog # QBCA-1KT) • Protein Standard (Sigma-Aldrich, catalog #PO914-10AMP) • Acrylamide gels 12.5% • Laemmli buffer 4X • Molecular weight precision plus protein standards (BIO-RAD, catalog #161-0375) • KimTech Science (Kimberly-Clark Professional) • PVDF membranes for protein blotting (BIO-RAD, catalog #1620177) • Ponceau S solution (Sigma-Aldrich, catalog #P7170-1L) • β -Actin (8H10D10) mouse monoclonal antibody (Cell Signaling Technologies, catalog #3700) • Bcl2 (EPR17509) rabbit monoclonal antibody (Abcam, catalog # ab182858) • eNOS mouse monoclonal antibody (BD Biosciences, catalog # 610296) • Perilipin A rabbit polyclonal antibody (Abcam, catalog # ab3526) • Histone H3 (C-16) goat polyclonal antibody (Santa-Cruz, catalog #sc-8654) • Goat Anti-Mouse IgG (H + L)-HRP Conjugate (BIO-RAD, catalog #1706516) • Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (BIO-RAD, catalog #1721019) • Donkey Anti-Goat IgG (H&L)-HRP (Abcam, catalog #ab97110) • Goat Anti-Mouse IgG (H + L)-HRP Conjugate (BIO-RAD, catalog #1706516) • Clarity Western ECL Substrate (BIO-RAD, catalog #170-5061) • ImageQuant LAS 4000 (GE Healthcare) • ImageJ image processing program (U.S. National Institutes of Health).

Techniques: Protein Extraction, Western Blot, Lysis, Mass Measurement, Protease Inhibitor, Centrifugation, Transferring, Sample Prep, Molecular Weight